Protein Estimation Methods
Tuesday, February 19, 2019
Determination of protein concentration is necessary and widely used in protein biology, molecular biology, and other research applications. The concentration of protein samples have to be estimated before proceeding to isolation, puriﬁcation, and analysis. Various protein estimation methods ... ..
Host Cell Protein Analysis: Detection in Biotherapeutics
Tuesday, February 12, 2019
Host cell proteins (HCPs) are one of the main impurities in bio-therapeutics that are carried over from the host expression system. Although HCPs are at low levels, they can trigger immunogenic reactions in the human system when administered along with bio-therapeutics. The similarity of physicoc... ..
Spotting Fragmentation Patterns When Using Mass Spectrometry
Tuesday, February 5, 2019
Fragmentation occurs when energetically unstable molecular atoms dissociate (either through homolytic or heterolytic cleavage) as they pass through the ionization chamber of a mass spectrometer. Here, the sample is bombarded with a stream of electrons powerful enough to remove an electron from th... ..
Tips for Preventing Protein Aggregation & Loss of Protein Solubility
Tuesday, January 29, 2019
Maintaining the stability and solubility of target proteins before, during and after purification is one of the biggest challenges researchers often run into. Proteins are extremely sensitive to solution conditions (i.e., pH and conductivity) and temperature. They also tend to aggregate at high c... ..
How to set up a teaching biotech lab
Wednesday, January 16, 2019
Congratulations, your hard work and the powers that be have allowed you to start a biotech program and set up your very own biotechnology teaching lab. Where do you even begin? ..
How to Lyse Bacterial Cells
Tuesday, January 15, 2019
How do you break open bacterial cells in the lab? While the enzyme lysozyme is mainly responsible for lysing bacterial cells in nature, you can achieve the same effect by using particular enzymes, detergents, and chaotropic agents, and/or certain mechanical methods (e.g., sonication, repeated fre... ..
Why Start a High School Biotech Course?
Wednesday, January 9, 2019
B iotechnology is one of the fastest growing industries and offers healthy starting salaries. Now is the time to educate students in biotechnology and the skills needed to pursue a career in this fast-growing field. ..
A Quick Guide to Choosing Fluorescent Dyes for Protein Labeling
Tuesday, January 8, 2019
Aside from its significant role in the natural world, fluorescence is also hard at work in biotechnology and the life sciences. In fact, fluorescent dyes are being widely used in numerous applications such as flow cytometry, immunofluorescence, Western blotting, and DNA electrophoresis. Just how ... ..
Biotech Education: Good for Students, Teachers & All
Friday, December 21, 2018
What is biotechnology, who uses biotechnology, and what does biotech give us? Quite simply, biotechnology is the application of biology, chemistry, and engineering research and manufacturing techniques to produce products and services to improve the quality of human life. ..
TOP 5 REASONS FOR A COLLEGE BIOTECH PROGRAM
Wednesday, December 19, 2018
5. Fastest Growing Industries: The Bureau of Labor Statistics (2017) is projecting a faster than average 11% growth in the biotechnology job market through 2026. The “in-demand” biotechnology jobs are Biomedical Engineers (7% growth), Biochemists (11% growth), Medical Scientist (13% growth), Biol... ..