A guide to in-vitro cultures: which matrix to be considered
Tuesday, October 16, 2018
In in-vivo systems, the cells have mechanical support in extracellular vicinity that not only provides overall stability but also facilitates cell-cell interaction, adhesion, cell proliferation, migration and differentiation. This mechanical support is a complex functional bioactive scaffold call... ..
Loop Mediated Isothermal Amplification (LAMP)
Tuesday, October 9, 2018
Loop mediated isothermal Amplification (LAMP) technique is one of the recent PCR techniques that is gaining importance for its application in molecular diagnostics. LAMP is a single tube PCR reaction carried out at constant temperature. LAMP is simple, affordable, cost effective, requires simple ... ..
Genome-wide Ribosome footprint profiling
Tuesday, October 2, 2018
There has been an exponential interest developing from past few years for studying the translational regulation of different genes in many model systems. Protein synthesis is the functional and fundamental biological process essential for decoding the genetic information and culminates it with a ... ..
An Overview of Histology Fixatives
Tuesday, September 25, 2018
Whenever you work in the histology lab, there is a great chance that you’ll be using some type of fixatives before you can proceed with your experiment. By definition, fixation is the process of preserving biological tissues by terminating any biochemical reactions thereby preventing autolysis an... ..
Why teach biotechnology in schools?
Monday, September 24, 2018
Biotechnology is the fastest growing occupation among industries, expected to grow 11% by 2022. Teaching biotechnology through the applications of biology, chemistry, engineering research, and manufacturing techniques is critical to the future of creating products and services that improve t... ..
Methods of Determining Protein Stability
Tuesday, September 18, 2018
The stability of proteins is crucial in many in vitro protein studies and is considered a major requirement in functional studies involving native and recombinant proteins . Thus, understanding protein stability and preserving the native conformation and normal functions of your protein of intere... ..
The Advantages of Using Trypsin for Mass Spectrometry
Tuesday, September 11, 2018
In mass spectrometry-based proteomics, nothing comes close to trypsin in breaking down protein mixtures into peptide fragments. In fact, protein researchers consider trypsin as the runaway winner when it comes to protease activity and specificity – and there are a lot of good reasons why th... ..
Antibody Labeling: Which Method Should You Use?
Tuesday, September 4, 2018
When it comes to labeling your antibodies for downstream application requiring signal detection, you have two choices. You can either go for the direct approach or the indirect approach. How do you know which method to use? Here are some things you need to know to make sure you pick the right met... ..
Protein Tags: How to Choose?
Tuesday, August 28, 2018
Expression of recombinant proteins with peptide or protein tags is widely used in protein research for three main reasons, ease of purification from a large pool of host proteins, enhancing solubility of the protein and for localization studies. Some important steps to be considered while choosin... ..
8 Techniques to Clone A Gene: Which Method is THe BEst FOr You?
Tuesday, August 21, 2018
Gene cloning is one of the most important steps in recombinant DNA Technology. Now-a- days researchers are using different cloning techniques depending on purpose, time, cost, ease of use and availability of resources. A few of the techniques are briefly explained below. ..