Best Methods to Stain Proteins After Protein Electrophoresis
Tuesday, June 18, 2019
Protein samples are analyzed by denaturing sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Total protein lysates or protein samples from purification experiments are separated on 1D and 2D gels depending on their molecular weights and PI. Appropriate staining techniqu... ..
Dyes, Ions, or Fluorescent Stains: What Are the Best Ways to Visualize Protei...
Tuesday, May 28, 2019
While researchers use several ways to visualize their protein samples, there isn’t a single best approach that is ideal for all cases. Each visualization technique has its own unique advantages and limitations, and so may only be suitable for certain applications. ..
Best Ways to Remove Detergents in Protein Samples
Tuesday, May 21, 2019
While detergents play a vital role in separating proteins from the hydrophobic portions of the cell membrane during extraction and sample preparation, they need to be removed after successfully serving their purpose to prevent them from interfering with downstream applications (e.g., IEF, ELISA, ... ..
Different Types of Extraction Buffers and When to Use Them
Tuesday, May 14, 2019
Successful biochemical analysis heavily relies on the effective extraction of biologically active proteins from source materials (e.g., cell and tissue samples). Thus, you need to have an excellent working knowledge of your target protein(s) and use the appropriate extraction buffers for a given ... ..
Preventing Carbamylation When Using Urea as a Protein Solubilization Buffer
Tuesday, May 7, 2019
When it comes to solubilizing and denaturing proteins prior to isoelectric focusing and 2-D gel electrophoresis, most researchers choose urea. This is not surprising since this mild chaotropic agent can completely disrupt the interactions between protein molecules and increase the efficiency of p... ..
8 Factors to Consider when Selecting a Protein Cross-linker
Tuesday, April 30, 2019
Protein cross-linking reagents, fondly called “cross-linkers” by researchers, are molecules with two or more reactive ends that can attach themselves to specific functional groups (e.g., primary amines, carboxyls, sulfhydryls, carbohydrates, and carboxylic acids) on proteins and other biomolecule... ..
BCA or Bradford Protein Assay: Choosing Between the Two
Tuesday, April 23, 2019
Which protein assay should you choose for your experiment – the BCA ( Bicinchoninic Acid) protein assay or the Bradford protein assay ? Since there is practically not a single protein assay method that is perfectly specific to particular proteins or sensitive to all protein types, your succ... ..
Four Methods for Endosomal Isolation
Tuesday, April 16, 2019
As a functional unit of a living entity, cells are very complicated and consist of exceptionally high number of biomolecules with completely different physical and chemical properties. The complexity of the biomolecules profile is even more intricately woven depending upon their subcellular locat... ..
Molecular Beacons: Dual Labeled Probes
Tuesday, April 9, 2019
Transcript analysis and DNA information is a crucial step in the field of molecular biology and genetics. For many years, DNA hybridization is utilized as a primal technique for diagnosis of genetic manipulations and their correlation with the diagnostic manifestations of the genetic disease... ..
Mycoplasma Contamination in Cell Cultures: Detection & Prevention
Tuesday, April 2, 2019
Cell culture is extensively growing technique applied in various fields of basic research, regenerative medicines and biotechnological applications. The use of cells is exponentially increasing in wide-range of studies involving cell division, cell growth and proliferation, bioassay development a... ..