Bradford Assay for Protein Concentration
Tuesday, December 3, 2019
The Bradford assay is a fast and efficient colorimetric assay to determine the protein concentration in a wide range of solutions and is widely used for protein content of cell fractions and protein concentrations for gel electrophoresis. It is often done before an SDS-PAGE analysis. Using the re... ..
Purify Proteins Fast: How FPLC Speeds the Process
Tuesday, November 26, 2019
Many researchers use high pressure liquid chromatography (HPLC) to separate and measure concentrations of small molecules. However, this method is relatively ineffective when trying to extract proteins and other biochemicals. Organic solvents used in HPLC place restrictions on these biochemicals,... ..
The Benefits of a Protease Inhibitor Cocktail
Tuesday, November 5, 2019
In a cell, protein degradation and synthesis maintain the balance of proteins, where old proteins are degraded into amino acids, which are further used in the synthesis of new proteins. Proteases/Peptidases play an important role in protein degradation. Proteases are present in almost all l... ..
Protein palmitoylation and sulfhydryl chemistry methods to capture palmitoyla...
Tuesday, October 29, 2019
Proteins undergo many kinds of post translational modifications (PTMs), such as phosphorylation, ubiquitination, SUMOylation, geranyl-geranylation, farnesylation, myristoylation, acetylation, succinylation and palmitoylation. PTMs can be reversible or irreversible, they can be dynamic or stable. ... ..
Complete Protease Inhibitor Cocktails and How They Work
Tuesday, October 22, 2019
What is a Protease Inhibitor? If you want to protect your target protein from potential harm, there is one thing you should do – use a protease inhibitor . ..
The Difference Between the BCA and Bradford Protein Assays
Tuesday, October 15, 2019
When conducting an experiment, it is essential to choose the correct protein assay. ..
Protein Kinases and Phosphatases: drivers of phosphorylation and dephosphoryl...
Tuesday, October 8, 2019
Proteins are phosphorylated and dephosphorylated all the times in the cells in a highly regulated manner. Protein phosphorylation and dephosphorylation are critical for signaling, cell division, protein translation, metabolism and survival. Activity of substrate proteins is tightly regulated by c... ..
Protein phosphorylation: Nature’s favorite way of decorating proteins
Tuesday, October 1, 2019
Part 1: Essentials of protein phosphorylation American chemist Westheimer asked an interesting question in 1987 in his very famous article published in ‘Science’ the same year, ‘why nature chose phosphates?’. He elegantly approached this problem from a chemists’ perspective. Phosphorus, a group 1... ..
Click chemistry and its application to proteomics
Tuesday, September 24, 2019
Nature has ability to generate an unending array of molecules from very small number of building blocks, even simple microbes such as E. coli have synthetic chemical prowess that can bring the most sophisticated organic chemistry labs to their shame. Inspired by the nature’s ability to knit compl... ..
Why Is Bovine Serum the Preferred Standard for Protein Assays?
Tuesday, September 10, 2019
Protein assays are one of the most widely used scientific methods, particularly in life sciences. An assay is an efficient spectroscopic procedure that analyzes the concentration of protein in a solution, also referred to as protein concentration quantitation. Being able to measure this concentra... ..