SILAC: A General Workflow for Improved Mass Spectrometry
Friday, April 13, 2018
This is a follow up blog to SILAC for Improved Mass Spectrometry & Quantitative Proteomics SILAC (Stable Isotope Labeling with Amino Acids in Cell Culture) method of working is differentiated into two critical phases: an adaptation phase and an experimental phase. Before initiating the adapta... ..
SILAC for Improved Mass Spectrometry & Quantitative Proteomics
Friday, April 13, 2018
SILAC (Stable Isotope Labeling with Amino Acids in Cell Culture) Quantitative proteomics has been a cutting-edge and demanding field in biological research for the past few decades. With the advancement of technology, MS (Mass spectrometry) based methods have become strikingly helpful tools, whic... ..
Key Differences of H&E and Special Stains for Immunohistochemistry
Wednesday, March 28, 2018
Routine H&E (Haemotoxylin and Eosin stains) and special staining comes especially handy when examining tissue structure and cell types and/or when looking for the presence of certain microorganisms in a sample. While both stains are used in histopathology laboratories, H&E stain is more c... ..
Study Protein to Protein Interaction with Protein Cross Linking to Glass
Friday, March 23, 2018
In all the complex biological processes, the mechanism underlies a synchronized and orchestrated molecular organization, which works through protein-protein interactions. These interactions could be transient, such as catalytic or signal-transduction pathways, requiring a transitory protein-... ..
When to Use Immunohistochemistry (IHC) Detection vs. a Direct Method
Tuesday, March 6, 2018
How do you know which method to use for visualizing proteins after immunodetection? Should you be using the direct detection method or would applying an indirect detection method best serve your purpose? If you’re not sure which method to use, here are some things you definitely need to know. ..
Traction Force Microscopy for cell interaction with extracellular matrix
Tuesday, February 27, 2018
The fundamental source of all processes in living system is force. Force is required to maintain the balance between the external and internal environment of any living system. Cells have the ability to sense and respond to the forces. Cells perform different activities such as migration and inte... ..
Immunoprecipitation Protocol: Critical Parameters
Tuesday, February 13, 2018
Following up the Immunoprecipitation Protocol: The Basics , we explore the IP protocol from the viewpoint of some of the protocols critical parameters. Antigen extraction: As mentioned in the general protocol , cellular antigens are required to be extracted from a suitable source depending on the... ..
Immunoprecipitation Protocol: The Basics
Tuesday, February 6, 2018
The cardinal principle behind immunoprecipitation (IP) is the isolation of an antigen by interaction between the antigen and a specific antibody conjugated to a sedimentable matrix. Antigen source may vary from cells or tissues without labeling, radio labeled or tagged cells, labeled&nb... ..
MTT Assay for Cytotoxicity
Tuesday, January 30, 2018
Tetrazolium reduction assays are characterized by reduction of various tetrazolium analogs (MTT, MTS, XTT, WST-1, and INT) and quantification of the subsequent color change. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was originally developed as an alternative to ... ..
Protein Storage For Protein Stability And Less Protein Degradation
Tuesday, January 23, 2018
Protein Storage and Stability What are Proteins? As one of the multifarious macromolecules, proteins are complex and crucial for cellular functions. Proteins are polymers built of monomer subunits called amino acids connected by a specific type of covalent bond known as a peptide bond. The proper... ..