The Sandwich ELISA: Process and Practical Applications
Tuesday, June 19, 2018
The Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) is one of the most efficient laboratory procedures used in detecting the presence and measuring the concentration of a target antigen in a completely unknown sample. Its superior sensitivity and extremely robust nature makes it a great diagno... ..
How to Improve Spot Resolution During 2D-Electrophoresis
Tuesday, June 12, 2018
While several protein separation technologies have been developed in recent years, two-dimensional gel electrophoresis remains one of the most popular techniques used in the field of proteomics. This is not surprising since 2D PAGE is routinely used to accurately analyze the individual components... ..
Recombinant Protein Expression Systems: Pros & Cons
Tuesday, June 5, 2018
Recombinant production of proteins involves transfecting cells with desired gene in a DNA vector. The gene then translates into a protein using host cellular machinery. These expressed proteins can then be extracted by lysing the cells ans subsequent purification steps. Both Prokaryotic and Eukar... ..
Cold-Shock Keeps Recombinant Proteins out of Inclusion Bodies
Tuesday, May 22, 2018
The cloning and expression of proteins to product recombinant proteins is a hugely popular technique that allows for the production of copious quantities of protein that almost mimic native proteins. Although a popular and useful technique, one of the main reasons for its use can also be a drawba... ..
BONCAT (Bioorthogonal Non-canonical Amino Acid Tagging): a method to define t...
Tuesday, May 15, 2018
Living entities have a tightly regulated dynamic system, where majority of the energy expenditure goes for maintaining the homeostasis. Most of the biological processes such as growth, development, metabolism etc are regulated at the crucial control point of protein translation and degradation (r... ..
Basics of Using Cytology for Cancer Testing
Tuesday, May 1, 2018
Aside from tissue biopsy, cytology serves as an indispensable tool in screening and diagnosing cancer. In this technique, a cytological material is obtained from the patient, spread onto glass slides for microscopic examination, stained, screened for abnormalities and assessed prior to the issuan... ..
SILAC: A General Workflow for Improved Mass Spectrometry
Friday, April 13, 2018
This is a follow up blog to SILAC for Improved Mass Spectrometry & Quantitative Proteomics SILAC (Stable Isotope Labeling with Amino Acids in Cell Culture) method of working is differentiated into two critical phases: an adaptation phase and an experimental phase. Before initiating the adapta... ..
SILAC for Improved Mass Spectrometry & Quantitative Proteomics
Friday, April 13, 2018
SILAC (Stable Isotope Labeling with Amino Acids in Cell Culture) Quantitative proteomics has been a cutting-edge and demanding field in biological research for the past few decades. With the advancement of technology, MS (Mass spectrometry) based methods have become strikingly helpful tools, whic... ..
Key Differences of H&E and Special Stains for Immunohistochemistry
Wednesday, March 28, 2018
Routine H&E (Haemotoxylin and Eosin stains) and special staining comes especially handy when examining tissue structure and cell types and/or when looking for the presence of certain microorganisms in a sample. While both stains are used in histopathology laboratories, H&E stain is more c... ..
Study Protein to Protein Interaction with Protein Cross Linking to Glass
Friday, March 23, 2018
In all the complex biological processes, the mechanism underlies a synchronized and orchestrated molecular organization, which works through protein-protein interactions. These interactions could be transient, such as catalytic or signal-transduction pathways, requiring a transitory protein-... ..