The Most Basic Biotech Lab Equipment, Materials & Necessities
Thursday, December 13, 2018
G-Biosciences and Ellyn Daugherty have compiled a simple list of equipment and materials required for a new biotechnology teaching lab. When setting up a new lab, it is often quite daunting, as it is hard to budget and plan if you are unsure of the basic requirements for the lab. This blog... ..
Physical Disruption or Chemical Cell Lysis? How to Decide
Thursday, December 13, 2018
Cell lysis is an essential step in the extraction and purification of intracellular proteins, and the method that you use to disrupt your cell samples can determine the success and reproducibility of your downstream applications to a great extent. Keep in mind that one vital component of the star... ..
Organic Solvent Extraction of Proteins
Tuesday, December 11, 2018
Protein extraction from plant, animal, fungi, bacteria, etc is well known using different techniques. Many buffers are used to solubilize water-soluble proteins. There are few proteins which are insoluble and are rigid to be extracted by physical means or they lose activity when extracted by dena... ..
Specificity of Immunostaining methods: How to choose a control?
Thursday, December 6, 2018
Immunocytochemistry is one of the most commonly used methods for protein localization in the cells or tissues using specific antibodies . The overall credibility of the results from Immunocytochemistry depends on two factors: ..
Sample preparation for Mass spectrometric analysis
Tuesday, December 4, 2018
Mass spectrometric analysis is one of the important technique in protein biochemistry and peptide analysis. It is used to identify and analyze proteins and peptide samples. Care should be taken for sample preparation for mass spec analysis as the quality of data depends on the purity of sample ap... ..
Blue Native Polyacrylamide Gel Electrophoresis: A simple tool for characteriz...
Thursday, November 29, 2018
Proteomics is a complex field and it is exposed to major developments over the last few decades. A general proteomic experiment is a multi-step process, which involves modifications and analysis at every step depending on the experimental requirement. Crudely, these experiments have the following... ..
Strategies to stabilize aggregate-prone proteins in E.coli
Tuesday, November 27, 2018
Maintaining protein function and stability during the entire process of protein expression and purification is crucial. Though a lot of strategies are being implemented by many researchers, each protein has its own requirements for its stability. Some proteins are prone to aggregation, a small tr... ..
Tips for Selecting Carrier Proteins
Thursday, November 22, 2018
As you are no doubt aware, carrier proteins play a significant role in antibody production. While immunogenic peptides are generally considered to be good epitopes by themselves, they are too small and lack the additional motifs required to link with class II molecules and T-cell receptors. Thus,... ..
Role of additives in cell lysis
Tuesday, November 20, 2018
The main challenge during protein expression and purification is prevention of protein degradation. Whether it is an E.coli expression system or insect or mammalian, protein degradation should be minimized or avoided during purification of proteins. Protein expression at lower temperatures someti... ..
A Quick Overview of Phenol Extraction
Thursday, November 15, 2018
While phenol extraction is one of the commonly used methods in purifying DNA samples (i.e., removing proteins from cell lysates during DNA preparation), some people do not fully understand how and why it works. Read on to learn and appreciate how phenol extraction works. ..