How to Iodinate Proteins with Iodo-Gen®
Tuesday, August 13, 2019
1,3,4,6-tetrachloro-3α,6α-diphenylglycoluril (Chloroglycoluril), also known as Iodo-Gen®, is one of the best reagents used for iodination of proteins, hormones, antibodies, viruses, cell membranes, etc. This method was first developed by Fraker et al. This method of iodination with Iodo-Gen® is s... ..
Hot Start PCR: An Overview
Tuesday, August 6, 2019
The polymerase chain reaction is a widely used technique for amplification of DNA products for genetic studies, DNA fingerprinting, clinical diagnostics, forensics and many more. Non-specific amplification of PCR products and the formation of primer dimers are the major problems during a PCR reac... ..
Use of Chaperones in Recombinant Protein Expression
Tuesday, July 30, 2019
Proteins that form complexes tend to form aggregates during recombinant expression. So the best method is to co-express proteins with suitable partner protein. Chaperones are one of those proteins that help insoluble protein formation. Researchers observed the enhanced solubility of some proteins... ..
Using Western Transfer for Protein Detection: Some Commonly Used Methods
Monday, July 29, 2019
Question: How to detect protein using western blotting? ..
The Basics of Genomic DNA Extraction
Tuesday, July 23, 2019
Genomic DNA extraction is an important part of the process when it comes to studying DNA. Pure DNA, separated from the proteins and fluid of cells, is needed on its own in order to be used for various molecular applications. These analyses can include cloning, sequencing, electrophoresis, and fin... ..
Iodination of Proteins with Bolton-Hunter Reagent
Tuesday, July 9, 2019
In the radioimmunoassay, high specific radioactive antigens are used as tracers. Iodinated proteins are used to study hormonal interaction with target tissue and immunoglobulin metabolism. The chloramine-t procedure is the oldest method used to iodinate proteins and peptides directly with 125 I, ... ..
Multiplex PCR, Benefits over traditional PCR
Wednesday, June 26, 2019
Multiplex PCR is a technique used to amplify multiple target sequences in a single PCR reaction using multiple primer sets. Multiplex PCR was first reported by Chamberlain et al. in 1988. This method is used to detect deletions, polymorphisms, mutations, etc., This method is also used to detect d... ..
Best Methods to Stain Proteins After Protein Electrophoresis
Tuesday, June 18, 2019
Protein samples are analyzed by denaturing sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Total protein lysates or protein samples from purification experiments are separated on 1D and 2D gels depending on their molecular weights and PI. Appropriate staining techniqu... ..
Dyes, Ions, or Fluorescent Stains: What Are the Best Ways to Visualize Protei...
Tuesday, May 28, 2019
While researchers use several ways to visualize their protein samples, there isn’t a single best approach that is ideal for all cases. Each visualization technique has its own unique advantages and limitations, and so may only be suitable for certain applications. ..
Best Ways to Remove Detergents in Protein Samples
Tuesday, May 21, 2019
While detergents play a vital role in separating proteins from the hydrophobic portions of the cell membrane during extraction and sample preparation, they need to be removed after successfully serving their purpose to prevent them from interfering with downstream applications (e.g., IEF, ELISA, ... ..