8 Techniques to Clone A Gene: Which Method is THe BEst FOr You?
Tuesday, August 21, 2018
Gene cloning is one of the most important steps in recombinant DNA Technology. Now-a- days researchers are using different cloning techniques depending on purpose, time, cost, ease of use and availability of resources. A few of the techniques are briefly explained below. ..
Post Translational Modifications: what expression system to choose?
Tuesday, August 14, 2018
Recombinant proteins are useful for studying biological processes and the structure of protein. Selecting an appropriate expression system is a crucial factor to produce correctly folded protein. Factors to consider when choosing an expression system include: ..
The Advantages of Biotinylation Tagging in Protein Purification
Tuesday, August 7, 2018
Biotinylation, also known as biotin tagging/labeling, is the process of attaching biotin to a protein molecule and other macromolecules. Considering its high affinity to avidin and streptavidin (with a dissociation constant of around 10 -15 M, biotin-avidin/streptavidin interaction is one of the ... ..
The Basics of Affinity Purification/Affinity Chromatography
Tuesday, July 31, 2018
Affinity purification, also called affinity chromatography, is a laboratory technique used for purifying protein or protein complexes within a biochemical mixture. Unlike other chromatography-based purification methods which separate molecules based on size (i.e., gel filtration or size-exclusion... ..
Restriction Enzyme Analysis: How to Make the Cut
Friday, July 27, 2018
A restriction enzyme (restriction endonuclease) is a special enzyme that recognizes a specific sequence of nucleotides and cleaves DNA at that specific site (restriction site or target sequence). These enzymes, which are usually found in bacteria and other prokaryotes, are considered as one... ..
Proteomic Grade Detergents: Why you should use them
Tuesday, July 17, 2018
Detergents play an extremely critical role in cell lysis and protein extraction. With their unique amphipathic nature (they possess a polar head group on one end that interacts with the hydrogen bonds of water molecules and a long hydrophobic carbon tail which aggregate to form micelles on the ot... ..
Exosome isolation by centrifugation, filtration, immunoaffinity & more
Tuesday, July 10, 2018
Earlier designated as the "garbage bags" used by the biological systems to remove unnecessary biomolecules of the cells, Exosomes , have recently gained popularity as secreted vesicles pivotal for intracellular and intercellular information transfer. Studies have shown the presence of essential R... ..
Tuesday, July 3, 2018
Diagnostic field has been a progressing area with multiple tools identified for specific proteins detection and their quantification. Diagnosis in pathology or clinical set up has multiple numbers of challenges owing to time constraint, increasing numerosity and complexity of the detection method... ..
Protein Solubility & Refolding Active Proteins from Inclusion Bodies
Tuesday, June 26, 2018
Over expression of recombinant protein in bacteria can lead to the accumulation of insoluble protein, which aggregates and is sequestered to inclusion bodies. Slow growth rate and low temperatures during protein expression will help in solubility of many over expressed proteins, however some prot... ..
The Sandwich ELISA: Process and Practical Applications
Tuesday, June 19, 2018
The Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) is one of the most efficient laboratory procedures used in detecting the presence and measuring the concentration of a target antigen in a completely unknown sample. Its superior sensitivity and extremely robust nature makes it a great diagno... ..