ECL (Enhanced Chemiluminescence) Reagents: Enhanced How?
Tuesday, April 25, 2017
There are a lot of reasons why researchers all over the world choose enhanced chemiluminescence (ECL) detection for a wide range of Western blot applications. ECL is considered as the experts’ detection method of choice due to its high sensitivity, excellent signal-to-noise ratio, and wide dynami... ..
Key Protein Concentration & Enrichment Techniques | G-Biosciences
Tuesday, April 18, 2017
What is protein enrichment and why is there a need for it? Basically, protein enrichment is a technique where proteins of interest in a biological sample are concentrated to make them more suitable for identification and downstream analysis. ..
Importance of detergent micelle levels in membrane protein purification
Tuesday, April 11, 2017
Detergents form micelles which can trap hydrophobic molecules into these micelles and allow the extraction of membrane proteins through solubilization. The “Critical Micelle Concentration” or CMC of a detergent is the concentration of a detergent in which micelles start to form. ... ..
Epoxy-activated resin: a versatile affinity chromatography support
Tuesday, April 4, 2017
Affinity chromatography is the most popular and widely used method for purification of biomolecules including proteins, antibodies, lectins, carbohydrates, nucleic acids and glycoproteins. Affinity purification is based upon specific surface interactions among biological molecules such as antigen... ..
Plant Protein Extraction and Protein Precipitation Techniques
Tuesday, March 28, 2017
Extracting proteins from plant and animal sources are two different things. While animal proteins can easily be extracted, working with plant proteins can be quite a challenge. Why? Plant proteins are more difficult to extract since they are protected by a rigid cell wall that contains several in... ..
Specific plant tissue sample preparation & protein extraction methods
Tuesday, March 21, 2017
As mentioned in our earlier posts , extracting, separating and identifying proteins from plant samples present a unique challenge since they contain significant amounts of proteases and other compounds that may interfere with the process. Generally, there are three critical aspects for successful... ..
Cell Disruption Techniques: Sonication, Dounce Homogenizer & More
Tuesday, March 14, 2017
Cell disruption is a collection of techniques used for releasing biomolecules of interest from inside the cell. This blog will discuss the various techniques which will be broken down into two categories, gentle and harsh cell disruption, there relative advantages and which tissue types wor... ..
Non-Specific Binding: Why It Needs to be Blocked in Western blots!
Tuesday, March 7, 2017
After running your gel and transferring your proteins to your membrane, there is one thing left for you to do to ensure the accurate analysis of your protein. You need to block all unoccupied sites on your membrane to prevent the non-specific binding of antibodies and other detection agents to yo... ..
Precautions with SDS Stock Solutions Preparation | G-Biosciences
Tuesday, February 28, 2017
Sodium dodecyl sulfate or SDS , is an anionic surfactant (detergent) commonly used in research lab due to its multiple functions. ..
Western Blot Blocking: Tips and Tricks
Wednesday, February 22, 2017
Western blotting is an immunodetection technique used to first separate proteins by gel electrophoresis then to transfer those separated proteins from the gel matrix onto a secondary, more durable matrix, most commonly polyvinylidene difluoride (PVDF) or nitrocellulose. ..