Four Methods for Endosomal Isolation
Tuesday, April 16, 2019
As a functional unit of a living entity, cells are very complicated and consist of exceptionally high number of biomolecules with completely different physical and chemical properties. The complexity of the biomolecules profile is even more intricately woven depending upon their subcellular locat... ..
Molecular Beacons: Dual Labeled Probes
Tuesday, April 9, 2019
Transcript analysis and DNA information is a crucial step in the field of molecular biology and genetics. For many years, DNA hybridization is utilized as a primal technique for diagnosis of genetic manipulations and their correlation with the diagnostic manifestations of the genetic disease... ..
Mycoplasma Contamination in Cell Cultures: Detection & Prevention
Tuesday, April 2, 2019
Cell culture is extensively growing technique applied in various fields of basic research, regenerative medicines and biotechnological applications. The use of cells is exponentially increasing in wide-range of studies involving cell division, cell growth and proliferation, bioassay development a... ..
Antibody Selection for Immunohistochemistry
Tuesday, March 26, 2019
Antibodies have become a great tool for identifying and characterization of different proteins in different model systems using the technique of immunohistochemistry and Western blotting. The precision and sensitivity of these molecules allow us to develop a clear-cut picture of molecular cascade... ..
Protein Purification with IMAC
Tuesday, March 19, 2019
Protein puriﬁcation based on fusing peptide afﬁnity tag to recombinant protein is widely preferred due to its ease of use. Immobilized Metal Ion Afﬁnity Chromatography (IMAC) was based on the principle of binding of speciﬁc amino acid side chains of a protein to metal ions immobilized on a matrix... ..
A Guide to Sample Preparation for Immunofluorescence
Tuesday, March 12, 2019
Immunofluorescence is an essential and a powerful tool in molecular biology for the visualization of cellular structures, protein localization and intracellular processes. The resultant sample produced after immunofluorescence is analyzed using different microscopic methods such as confocal micro... ..
Detection of Apoptosis by TUNEL Assay
Tuesday, March 5, 2019
At a cellular level, there is always a homeostasis maintained between cell division and cell death. The process of growth and development involves an active balance of both of these mechanisms. Cell death occurs through three distinct mechanisms depending upon the molecular signal: Apoptosis, Nec... ..
Luciferase Reporter Assays: An Overview
Tuesday, February 26, 2019
Reporters have contributed significantly to the field of biology and its progress. Genetic reporters are widely used in drug development, biomedical research, and biochemistry. In a nutshell, the reporter gene is fused with the gene of interest and cloned into an expression vector. The expression... ..
Protein Estimation Methods
Tuesday, February 19, 2019
Determination of protein concentration is necessary and widely used in protein biology, molecular biology, and other research applications. The concentration of protein samples have to be estimated before proceeding to isolation, puriﬁcation, and analysis. Various protein estimation methods ... ..
Host Cell Protein Analysis: Detection in Biotherapeutics
Tuesday, February 12, 2019
Host cell proteins (HCPs) are one of the main impurities in bio-therapeutics that are carried over from the host expression system. Although HCPs are at low levels, they can trigger immunogenic reactions in the human system when administered along with bio-therapeutics. The similarity of physicoc... ..