Western Blot Blocking: Tips and Tricks
Wednesday, February 22, 2017
Western blotting is an immunodetection technique used to first separate proteins by gel electrophoresis then to transfer those separated proteins from the gel matrix onto a secondary, more durable matrix, most commonly polyvinylidene difluoride (PVDF) or nitrocellulose. ..
Cell Lysis: 5 Common Cell Disruption Methods | G-Biosciences
Tuesday, February 14, 2017
When working on proteins, your sample biological materials should first be homogenized to ensure proper solubilization and extraction. This can be a challenge if you are trying to extract plant proteins because their tissues contain significant amounts of proteases, phosphatases and glycosidases ... ..
Detergents: Ionic, Non-Ionic, and Zwitterionic. What's the Difference?
Wednesday, February 8, 2017
There is a plethora of uses for detergents, both in the lab and in everyday life. The usefulness of these cleaning compounds comes from subtle differences in their chemical structure. Detergents are composed of amphipathic molecules, containing a polar hydrophilic head group attached to a long-ch... ..
Buffer Solution Preparation: An Essential Skill for Researchers
Friday, February 3, 2017
Many high school and college students realize that having research experience in an academic or industry lab has many benefits. Many search for paid or unpaid internships, part-time employment or propose a research project in their instructor’s lab, working independently, applying what they alrea... ..
Plant vs Animal Cells: Break the Cell Wall with Lytic Enzymes
Tuesday, January 31, 2017
Extracting proteins from plants is not the same as extracting proteins from animal sources. There is a very different set of factors that you will need to consider in order to properly process plant proteins. This post explores some of the key ways in which plant-based protein extraction differs ... ..
Western blot: Constant current or constant voltage; which is best?
Friday, January 20, 2017
The choice of constant current or constant voltage will depend on several different factors that must be taken into consideration for each Western transfer test. The level of heat, duration of the test, type of proteins, the size of the transfer, and the rate of transfer will all be affected... ..
Best Blocking Buffer Selection for ELISA & Western Blot
Tuesday, January 10, 2017
Antibodies are used to detect specific proteins during research. There are a variety of tests including the Western blot, that produce a surface area that can contain other proteins and components that need to be blocked. Blocking buffers are used to bind the surface areas that may attach to reag... ..
Polyclonal Antibody: Advantage over Monoclonals?
Friday, January 6, 2017
Polyclonal antibodies are groups of antibodies that are able to identify many different epitopes of a particular antigen. The antibodies are derived from a selection of B cells, each one recognizing one particular epitope. A monoclonal antibody has one single source. Polyclonal antibodies are eas... ..
Viability Assays: Different Types and Their Use in Cell Death Research
Tuesday, November 22, 2016
Viability assays are strategies and techniques that are generally used to determine whether or not an organ, cell, or tissue remains viable or could potentially recover its viability. In general, viability assays will look at the cells or tissue involved to see if they still react and move as the... ..
What is Protein Electrophoresis and When Should It Be Used?
Tuesday, November 15, 2016
When proteins need to be segregated out from a sample, protein electrophoresis may be used. Protein electrophoresis utilizes a matrix and an electrical current in order to easily separate proteins without disruption. Protein electrophoresis is beneficial for a few reasons: it can be performed on ... ..