Remove BSA & Gelatin from Antibody Solutions for Antibody Labeling
Tuesday, January 9, 2018
Purified and commercial antibodies are routinely stored in buffers that contain the proteins bovine serum albumin (BSA) and gelatin as these act as stabilizers during long term storage. The presence of 0.2-1% (2-10mg/ml) BSA and/or gelatin help to stabilize antibody solutions that are ... ..
Bacterial Growth Curves using a Spectrophotometer (Turbidimetric Determination)
Tuesday, November 28, 2017
Bacteria are as interesting as they are diverse. Though tiny, these unicellular life forms make huge contributions to many systems and cycles. From helping break down food in your intestine; to making the molecular assist in all three of the carbon, phosphorus, and nitrogen cycles -- these little... ..
Speed Up Protein Purification with FastPure™ Spin Columns
Wednesday, November 15, 2017
Affinity chomatography is a routinely used technique for purifying or enriching a protein or molecule of interest through its specific binding affinity. The target protein adheres to a particular ligand that has been immobilized on a solid support (usually beaded agarose resin). This process prod... ..
Overcome effects of detergents & reducing agents in protein estimation
Tuesday, November 7, 2017
Accurate protein quantitation is an integral part of any laboratory workflow involving protein purification, electrophoresis, cell biology, molecular biology, and other research applications. Careful consideration must be taken when choosing the appropriate protein quantitation assay for a specif... ..
Chromatography Series Part I: Liquid Chromatography Basics
Tuesday, October 31, 2017
Chromatography is a versatile field with a wide range of applications. It’s accomplished by fractionating a mixture into its molecular components. Chromatography was first used in 1901 by Russian botanist Mikhail Tsvet when he realized the technique could separate plant pigments. It has since bec... ..
How to isolate membrane receptor complexes from cells
Tuesday, October 24, 2017
Membrane proteins play a key role in cellular processes including transport of molecules, signal transduction, utilization of energy and maintenance of cell and tissue structures. It has been determined by genome sequencing that around 30% of genes encode membrane proteins. Furthermore, they are ... ..
How much biotin is coupled to my protein? The HABA/Avidin Assay
Tuesday, October 17, 2017
Biotin, a 244 Dalton molecule, exhibits an extraordinary binding affinity for avidin and streptavidin (K a =10 15 M -1 ). This high binding affinity means that proteins, particularly antibodies, are routinely labeled with biotin. The labeled proteins can now be rapidly purified with av... ..
Sputum Processing, from viscous mucus to homogenous samples
Tuesday, October 10, 2017
Sputum Liquefaction: from viscous, heterogeneous mucus to equivalent, homogenous samples Sputum processing has one main purpose; to render the viscous, heterogeneous specimen to a homogenous state (evenly distributed suspension). This homogenous suspension will be dispensed into mu... ..
High Efficiency & Stability Protein CrossLinking with EDC & NHS
Tuesday, September 26, 2017
EDC is the most popular zero-length crosslinker for biochemical conjugations because it can efficiently form conjugates between two protein molecules, between a protein and a peptide, and between proteins and oligonucleotides, and with small molecules. ..