DNA Extraction: Difference in Plasmid and Genomic DNA Isolation
Tuesday, June 20, 2017
Isolating genomic and plasmid DNA for further investigation and downstream application (e.g. PCR, sequencing, etc.) requires totally different protocols. While isolating genomic DNA merely requires you to crack open the cell walls and purify the resulting sample, extracting plasmid DNA may be a b... ..
Mitochondrial DNA from Fungi (yeast) via Spheroplasts
Tuesday, June 13, 2017
INTRODUCTION When we speak of mitochondria, anyone with knowledge of life science could tell you of its presence in animal cells and absence in plant cells. However, it should not be forgotten that another kingdom shares this cellular powerhouse - kingdom fungi. ..
CNBr-activated resin to immobilize ligands for affinity chromatography
Tuesday, June 6, 2017
Commercially available affinity purification supports are designed on the principle of specific surface interactions among biomolecules, including, but not limiting to, antigen-antibody, enzyme-ligand and lectin-carbohydate. Affinity chromatography is one of the most efficient tools used for puri... ..
Western Blot Blocking: Tips and Tricks for Blocking Agents
Tuesday, May 30, 2017
Good Western blot blocking methods are crucial in order to achieve clean and reliable results. Protein samples are first ran on a polyacrylamide gel to separate proteins by size. The proteins are then transferred to a nitrocellulose or PVDF membrane. A blocking agent is then used to pre... ..
The Differences between a Protoplast and a Spheroplast (And Their Subsequent ...
Tuesday, May 16, 2017
Both protoplasts and spheroplasts refer to altered forms of plant, bacterial or fungal cells from which the cell wall has been partially or completely removed. These cells usually have all the other cellular components, except for the cell wall. When used in reference to bacterial cells, protopla... ..
Cellulase, a lytic enzyme to break plant cell walls for protein extraction
Tuesday, May 9, 2017
Lytic enzymes such as cellulase, lysozome , labiase, achropeptidase and a number of others, play a significant role in protein and DNA extraction. While different lytic enzymes can be used for different applications, cellulase is usually the enzyme of choice when it comes to breaking down plant c... ..
Stripping Membranes: A Great Addition to Western Blot Protocols
Tuesday, May 2, 2017
Commercial stripping buffers, such as G-Bioscience’s Western ReProbe™ and Western ReProbe™ PLUS , are specifically formulated to dissociate and remove all primary and secondary antibodies from the membrane-immobilized proteins without destroying the antigenic binding sites or removing the protein... ..
ECL (Enhanced Chemiluminescence) Reagents: Enhanced How?
Tuesday, April 25, 2017
There are a lot of reasons why researchers all over the world choose enhanced chemiluminescence (ECL) detection for a wide range of Western blot applications. ECL is considered as the experts’ detection method of choice due to its high sensitivity, excellent signal-to-noise ratio, and wide dynami... ..
Key Protein Concentration & Enrichment Techniques | G-Biosciences
Tuesday, April 18, 2017
What is protein enrichment and why is there a need for it? Basically, protein enrichment is a technique where proteins of interest in a biological sample are concentrated to make them more suitable for identification and downstream analysis. ..
Importance of detergent micelle levels in membrane protein purification
Tuesday, April 11, 2017
Detergents form micelles which can trap hydrophobic molecules into these micelles and allow the extraction of membrane proteins through solubilization. The “Critical Micelle Concentration” or CMC of a detergent is the concentration of a detergent in which micelles start to form. ... ..