Specific plant tissue sample preparation & protein extraction methods
Tuesday, March 21, 2017
As mentioned in our earlier posts , extracting, separating and identifying proteins from plant samples present a unique challenge since they contain significant amounts of proteases and other compounds that may interfere with the process. Generally, there are three critical aspects for successful... ..
Cell Disruption Techniques: Sonication, Dounce Homogenizer & More
Tuesday, March 14, 2017
Cell disruption is a collection of techniques used for releasing biomolecules of interest from inside the cell. This blog will discuss the various techniques which will be broken down into two categories, gentle and harsh cell disruption, there relative advantages and which tissue types wor... ..
Non-Specific Binding: Why It Needs to be Blocked in Western blots!
Tuesday, March 7, 2017
After running your gel and transferring your proteins to your membrane, there is one thing left for you to do to ensure the accurate analysis of your protein. You need to block all unoccupied sites on your membrane to prevent the non-specific binding of antibodies and other detection agents to yo... ..
Precautions with SDS Stock Solutions Preparation | G-Biosciences
Tuesday, February 28, 2017
Sodium dodecyl sulfate or SDS , is an anionic surfactant (detergent) commonly used in research lab due to its multiple functions. ..
Western Blot Blocking: Tips and Tricks
Wednesday, February 22, 2017
Western blotting is an immunodetection technique used to first separate proteins by gel electrophoresis then to transfer those separated proteins from the gel matrix onto a secondary, more durable matrix, most commonly polyvinylidene difluoride (PVDF) or nitrocellulose. ..
Cell Lysis: 5 Common Cell Disruption Methods | G-Biosciences
Tuesday, February 14, 2017
When working on proteins, your sample biological materials should first be homogenized to ensure proper solubilization and extraction. This can be a challenge if you are trying to extract plant proteins because their tissues contain significant amounts of proteases, phosphatases and glycosidases ... ..
Detergents: Ionic, Non-Ionic, and Zwitterionic. What's the Difference?
Wednesday, February 8, 2017
There is a plethora of uses for detergents, both in the lab and in everyday life. The usefulness of these cleaning compounds comes from subtle differences in their chemical structure. Detergents are composed of amphipathic molecules, containing a polar hydrophilic head group attached to a long-ch... ..
Buffer Solution Preparation: An Essential Skill for Researchers
Friday, February 3, 2017
Many high school and college students realize that having research experience in an academic or industry lab has many benefits. Many search for paid or unpaid internships, part-time employment or propose a research project in their instructor’s lab, working independently, applying what they alrea... ..
Plant vs Animal Cells: Break the Cell Wall with Lytic Enzymes
Tuesday, January 31, 2017
Extracting proteins from plants is not the same as extracting proteins from animal sources. There is a very different set of factors that you will need to consider in order to properly process plant proteins. This post explores some of the key ways in which plant-based protein extraction differs ... ..
Western blot: Constant current or constant voltage; which is best?
Friday, January 20, 2017
The choice of constant current or constant voltage will depend on several different factors that must be taken into consideration for each Western transfer test. The level of heat, duration of the test, type of proteins, the size of the transfer, and the rate of transfer will all be affected... ..