Immunoprecipitations (IP) for Protein Purification & Interactions Studies
Tuesday, August 15, 2017
ImmunoPrecipitation (IP) is a very useful immunochemical technique for isolating and purifying target proteins out of a complex sample (lysate). In conjunction with western blotting and/or other assay techniques, IPs can be used to determine: the presence and quantity of a protein; molecular weig... ..
How to select the Right protease inhibitor
Tuesday, August 8, 2017
While proteases are essential in maintaining the proper function of living cells, researchers often do not welcome their presence in their protein samples. Why would they when the mere presence of these enzymes jeopardizes the integrity of their samples and puts them one step closer to imminent d... ..
Protect proteins with cryoprotectants & protein concentration
Tuesday, August 1, 2017
Rough handling and proteins do not necessarily go well together. As proteins are extremely sensitive and can easily be degraded during the process of concentration, purification, and storage, they need to be handled with utmost care to reduce, if not eliminate, the risk of unwanted aggregation an... ..
ELISA Blocking Agents & Blocking Solutions
Tuesday, July 25, 2017
A blocking buffer is a solution of irrelevant protein, mixture of proteins, or other compound that passively adsorbs to all remaining binding surfaces of the plate. The blocking buffer is effective if it improves the sensitivity of an assay by reducing background interference and improving the si... ..
Optimize Antigen and Antibody Concentration for Western Blots
Tuesday, July 18, 2017
Advantages of Magnetic Beads for Protein Immunoprecipitation
Tuesday, July 11, 2017
While agarose beads have been traditionally used for immunoprecipitation (IP) and other micro-scale protein and antibody purification procedures, magnetic beads have since taken lead and are now considered tool for protein immunoprecipitation. ..
Detergents and their role in successful SDS-PAGE electrophoresis
Tuesday, July 4, 2017
SDS- P AGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) is a common laboratory technique in which proteins are separated by their size by running the proteins through a polyacrylamide matrix by applying an electrical field across the matrix. A native gel electrophoresis is when the... ..
Polyclonal Antibody Advantage for Antigen and Epitope Polymorphism
Tuesday, June 27, 2017
Monoclonal antibodies are often marketed as being superior to polyclonal antibodies. While this might be the case in many applications, this is not necessarily always true. In order to understand what type of antibody is best suited for a particular application, one needs to understand how they a... ..
DNA Extraction: Difference in Plasmid and Genomic DNA Isolation
Tuesday, June 20, 2017
Isolating genomic and plasmid DNA for further investigation and downstream application (e.g. PCR, sequencing, etc.) requires totally different protocols. While isolating genomic DNA merely requires you to crack open the cell walls and purify the resulting sample, extracting plasmid DNA may be a b... ..
Mitochondrial DNA from Fungi (yeast) via Spheroplasts
Tuesday, June 13, 2017
INTRODUCTION When we speak of mitochondria, anyone with knowledge of life science could tell you of its presence in animal cells and absence in plant cells. However, it should not be forgotten that another kingdom shares this cellular powerhouse - kingdom fungi. ..