When to Use Immunohistochemistry (IHC) Detection vs. a Direct Method
Tuesday, March 6, 2018
How do you know which method to use for visualizing proteins after immunodetection? Should you be using the direct detection method or would applying an indirect detection method best serve your purpose? If you’re not sure which method to use, here are some things you definitely need to know. ..
Traction Force Microscopy for cell interaction with extracellular matrix
Tuesday, February 27, 2018
The fundamental source of all processes in living system is force. Force is required to maintain the balance between the external and internal environment of any living system. Cells have the ability to sense and respond to the forces. Cells perform different activities such as migration and inte... ..
Immunoprecipitation Protocol: Critical Parameters
Tuesday, February 13, 2018
Following up the Immunoprecipitation Protocol: The Basics , we explore the IP protocol from the viewpoint of some of the protocols critical parameters. Antigen extraction: As mentioned in the general protocol , cellular antigens are required to be extracted from a suitable source depending on the... ..
Immunoprecipitation Protocol: The Basics
Tuesday, February 6, 2018
The cardinal principle behind immunoprecipitation (IP) is the isolation of an antigen by interaction between the antigen and a specific antibody conjugated to a sedimentable matrix. Antigen source may vary from cells or tissues without labeling, radio labeled or tagged cells, labeled&nb... ..
MTT Assay for Cytotoxicity
Tuesday, January 30, 2018
Tetrazolium reduction assays are characterized by reduction of various tetrazolium analogs (MTT, MTS, XTT, WST-1, and INT) and quantification of the subsequent color change. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was originally developed as an alternative to ... ..
Protein Storage For Protein Stability And Less Protein Degradation
Tuesday, January 23, 2018
Protein Storage and Stability What are Proteins? As one of the multifarious macromolecules, proteins are complex and crucial for cellular functions. Proteins are polymers built of monomer subunits called amino acids connected by a specific type of covalent bond known as a peptide bond. The proper... ..
Is Your BCA Protein Assay Really the Best Choice?
Tuesday, January 16, 2018
The BCA assay ( bicinchoninic acid assay) developed by Paul K. Smith et al. has become one of the most widely used protein assays since its introduction in 1985. The advantages offered by the assay help to explain the popularity and widespread use over other protein assays. The advan... ..
Remove BSA & Gelatin from Antibody Solutions for Antibody Labeling
Tuesday, January 9, 2018
Purified and commercial antibodies are routinely stored in buffers that contain the proteins bovine serum albumin (BSA) and gelatin as these act as stabilizers during long term storage. The presence of 0.2-1% (2-10mg/ml) BSA and/or gelatin help to stabilize antibody solutions that are ... ..
Bacterial Growth Curves using a Spectrophotometer (Turbidimetric Determination)
Tuesday, November 28, 2017
Bacteria are as interesting as they are diverse. Though tiny, these unicellular life forms make huge contributions to many systems and cycles. From helping break down food in your intestine; to making the molecular assist in all three of the carbon, phosphorus, and nitrogen cycles -- these little... ..
Speed Up Protein Purification with FastPure™ Spin Columns
Wednesday, November 15, 2017
Affinity chomatography is a routinely used technique for purifying or enriching a protein or molecule of interest through its specific binding affinity. The target protein adheres to a particular ligand that has been immobilized on a solid support (usually beaded agarose resin). This process prod... ..